A new Optogenetic Tool, a protein that can be controlled by light has been characterized by researchers at Ruhr- University Bochum (RUB). They used an opsin – a protein that occurs in the brain and Eyes – from Zebrafish and introduced it into the brain of mice unlike other Optogenetic tools, this opsin is not switched on but rather switched off by light. The experiment also showed that the tools could be suitable for investigating changes in the brain that are responsible for the development of Epilepsy.
The teams led by Professor Melanie Mark from the Behavioral Neurobiology Research Group and Professor Stefan Herlitze from the Department of General Zoology and Neurobiology describe the experiment and results in the Journal Nature of Communication.
The role assumed in various conditions:
The opsin Opn7b is a G protein-coupled receptor that is found in Zebrafish unlike the receptor, it can be activated without a light stimulus and is this permanently active. Normally, activation of G protein-coupled receptors leads to an opening of certain ion channels and thus to the influx of ions into the cell as well as to further signalling processes in the cell. In the case of Opn7b, light deactivates this permanently active signalling chain.
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Little research has so far been conducted on G protein-coupled receptors that are activated without stimulation, although it is presumed that they play a role in various neuropsychiatric conditions and night blindness they also appear to be involved in the development of virally induced Cancers.
Receptor characterized more precisely:
Dr. Raziye Karapinar, Dr. Siveke and Dr. Dennis Eickellbeck characterized the function of Opn7b in detail and to their surprise, identified that the receptor is deactivated by light. In contrast, conventional Optogenetic tools are switched on by light.
The researchers consider Opn7b well-suited to gain further insights into the function of G protein-coupled receptors that are constitutively active and obtain new knowledge of their role in the department of diseases in which the receptors can be examined in a time-controlled manner in specific cell types.
The Bochum researchers Dr. Jan Claudius Schwitalla and Johanna Pakusch changed certain cells in the cerebral cortex of mice in such a way that they produced Opn7b. If they deactivate the receptor with light, it triggered epileptic activity in the animals, which could be specifically controlled with light and interrupted with the help of other light-controlled proteins. The researchers hope that it will be possible to use these optogenetic tools to understand more precisely both the underlying mechanisms and the timescales in the development of epileptic seizures.
By: Peace Chigozie